BIO09004 2008 PROTEIN PURIFICATION PROCESSING

General Details

Full Title
PROTEIN PURIFICATION PROCESSING
Transcript Title
PROTEIN PURIFICATION PROCESS
Code
BIO09004
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
ASCI - Applied Sciences
Level
09 - NFQ Level 9
Credit
05 - 05 Credits
Duration
Stage
Fee
Start Term
2008 - Full Academic Year 2008-09
End Term
9999 - The End of Time
Author(s)
Kieran Tobin
Programme Membership
SG_SBIOP_E09 200900 Certificate in Biopharmaceutical Science SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_E09 201700 Level 9 Certificate in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIOP_O09 201700 Postgraduate Diploma in Science in Biopharmaceutical Science SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIPR_M09 201900 Master of Science in Biopharmaceutical Processing
Description

This module examines all downstream processing aspects of a typical biopharmaceutical production process. It includes the various design factors that need to be considered for the design of an effective and efficient protein capture and purification process. 

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Design an effective and efficient  downstream process for a protein capture and purification application.

2.

Outline and evaluate the various approaches to primary capture of the target material focusing primarily on affinity chromatography. 

3.

Describe the theory and practice of chromatography for purification applications.

4.

Outline and evaluate the various chromatographic techniques available for purification and their different applications including relative advantages and disadvantages.

5.

Interpret and explain other potential downstream processing technologies such as microfiltration, ultrafiltration, diafiltration,  centrifugation, electrophoresis and viral exclusion techniques. 

6.

Outline and evaluate the main quality control and analytical techniques required for downstream processing.

7.

Describe and critically evaluate the yield calculations for the various purification steps and their implications for overall process economics.

Indicative Syllabus

The range of topics covered will include some of the following: intracellular vs. extracellular protein in cell culture; the principles and operation of centrifugation; microfiltration, ultrafiltration, tangential flow filtration, diafiltration; electrophoresis; chromatographic techniques including gel filtration, ion-exchange (AEX and CEX), hydrophobic interaction, affinity-based techniques for both primary capture and protein purification; fluidised bed vs. packed bed design and column scale-up implications; yield calculations for chromatography steps; HETP and asymmetry in chromatography; viral exclusion technologies; quality control and analytical techniques for downstream processing.    

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
100 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc. Continuous Assessment UNKNOWN 100 % OnGoing 1,2,3,4,5,6,7
             
             

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Not Specified Lecture 2 Weekly 2.00
Independent Learning UNKNOWN Self Study 7 Weekly 7.00
Total Part Time Average Weekly Learner Contact Time 2.00 Hours

Module Resources

Non ISBN Literary Resources

Walsh, Gary, Biopharmaceuticals., Wiley, 2003

Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988

Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.,

Amersham-Pharmacia. (www.amersham.com), 2000

Other Resources

None