BIO09002 2008 FERMENTATION AND CELL CULTURE PROCESS

General Details

Full Title
FERMENTATION AND CELL CULTURE PROCESS
Transcript Title
FERMENTATION AND CELL CULTURE
Code
BIO09002
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
ASCI - Applied Sciences
Level
09 - NFQ Level 9
Credit
05 - 05 Credits
Duration
Stage
Fee
Start Term
2008 - Full Academic Year 2008-09
End Term
9999 - The End of Time
Author(s)
Kieran Tobin
Programme Membership
SG_SBIOP_E09 200900 Certificate in Biopharmaceutical Science SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIOP_E09 201700 Level 9 Certificate in Biopharmaceutical Science SG_SBIOP_O09 201700 Postgraduate Diploma in Science in Biopharmaceutical Science SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIPR_M09 201900 Master of Science in Biopharmaceutical Processing
Description

This module examines all upstream processing aspects of a typical biopharmaceutical production process. It includes the various design factors that need to be considered for bioprocess design.  The primary focus will be on recombinant mammalian cell culture lines with some aspects of microbial lines also included.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Identify and evaluate key factors that are involved in the design of an upstream bioprocessing system including which cell line to choose for a particular processing application and the cell-line storage conditions - cell banking and cell culture scale-up.

2.

Evaluate and discuss the various designs of bioreactors available and their key features including relative advantages and disadvantages. Also describe the different options for media feeding systems.

3.

Outline and evaluate the process kinetics relating to cell growth and productivity for a bioreactor process.

4.

Identify and interpret the basic mass transfer details for heat, oxygen and carbon dioxide - also the mass and energy transfer balances within a bioreactor process.

5.

Discuss and critically evaluate the different types of media available and their relative advantages and disadvantages.

6.

Evaluate and communicate the concepts of product yield and process productivity and their implications for overall process economics - how to optimise a bioreactor process.

7.

Describe the main aspects of bioreactor control and operation including the various instrumentation types involved.

Indicative Syllabus

The range of topics covered will include some of the following: cell line selection for optimal protein expression; cell banking and product scale-up; media selection and nutritional requirements; the process kinetics of cell growth and replication; the thermodynamic stoichiometric equations that apply for basic mass and energy transfer balances; basic mass transfer mechanisms for oxygen, carbon-dioxide and heat; bioreactor design types such as stirred tank reactors, fixed-bed, fluidized-bed, membrane and immobilized reactors; media feeding systems such as batch, fed-batch, continuous, continuous with cell recycle and perfusion; bioreactor instrumentation and controls both online and offline; product yield and productivity and their economic implications. 

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
100 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc. Continuous Assessment UNKNOWN 100 % OnGoing 1,2,3,4,5,6,7
             
             

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Not Specified Lecture 2 Weekly 2.00
Independent Learning UNKNOWN Self Study 7 Weekly 7.00
Total Part Time Average Weekly Learner Contact Time 2.00 Hours

Module Resources

Non ISBN Literary Resources

Walsh, Gary., Biopharmaceuticals, Wiley, 2003

Ratledge, Colin and Kristiansen Bjorn, Basic Biotechnology, Cambridge Press, 2001

Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002

Other Resources

None