FORS08012 2019 FORENSIC MOLECULAR BIOLOGY

General Details

Full Title
FORENSIC MOLECULAR BIOLOGY
Transcript Title
FORENSIC MOLECULAR BIOLOGY
Code
FORS08012
Attendance
N/A %
Subject Area
FORS - Forensic Studies
Department
LIFE - Life Sciences
Level
08 - NFQ Level 8
Credit
10 - 10 Credits
Duration
Semester
Fee
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Sharon Barrett
Programme Membership
SG_SFORE_K08 201900 Bachelor of Science (Honours) in Science in Forensic Investigation and Analysis SG_SFORE_H08 201900 Bachelor of Science (Honours) in Science in Forensic Science and Analysis
Description

This module aims to provide the student with specialised knowledge in the theory and practice of forensic DNA typing by investigating the biology, technology and genetic interpretation underpinning modern forensic DNA typing methods.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Demonstrate intellectual knowledge by explaining the principles underpinning modern forensic DNA typing.

2.

Demonstrate professional/practical skills by being able to perform and interpret selected practical applications of forensic DNA typing methods.

3.

Analyse, evaluate and draw conclusions from data obtained in the laboratory and/or data presented in problem solving exercises.

4.

Communicate scientific data in an appropriate format.

Teaching and Learning Strategies

This module will be delivered full‑time. Lecture delivery will be the primary mode of dissemination for module theory while practical applications will be explored during laboratory sessions.  Critical and reflective thinking will be developed through the assessment of laboratories which will involve report writing, data analysis, calculations and problem solving. This teaching and learning strategy will be augmented by both independent and directed learning strategies. Moodle will serve as the repository for educational resources (e.g., lectures, online resource information, access to journals etc) and also as a means of assessment (e.g. quizzes, uploading of lab reports, assignments, etc.).

 

Module Assessment Strategies

This module will be assessed using a combination of continuous assessment of theory [MCQ x2 (15%) around week 5 and week 9] and end of semester final exam to assess the theory content of the module (35%) plus laboratory practicals (50%). Laboratory skills, report‑writing and critical analysis of results will be assessed as part of the continuous assessment aspect of this module which will be examined through the submission of lab reports, data analytics including analysing electropherograms and sequencing chromatograms plus use of population databases and the product rule for estimation of random match probabilities (50%).

A minimum of 75% attendance at laboratory sessions is required. 

Repeat Assessments

Repeat continuous assessments, practical reports and/or final exam.

 

Module Dependencies

Prerequisites
None
Co-requisites
None
Incompatibles
None

Indicative Syllabus

LO1 Demonstrate intellectual knowledge by explaining the principles underpinning modern forensic DNA typing.

Explain the principles behind the different forensic DNA typing methods.

Sample collection methods.

Explain the range of techniques used for the extraction, quantification and analysis of trace DNA.

DNA separation and detection methods.

Explain the range of analytical techniques for DNA typing including; Polymerase Chain Reaction (PCR), Real-time PCR, Sequencing technologies plus new and emerging technologies .

Be able to discuss forensic issues such as Degraded DNA, PCR inhibition, contamination and low copy number.

Explain the theory of minisatellites or variable number tandem repeats (VNTRs), short tandem repeats (STRs), single nucleotide polymorphisms (SNPs).

Explain the theory and limitations of Nuclear profiling, mini-satellites, Y chromosome DNA testing, Mitochondrial DNA analysis plus emerging DNA profiling methods.

Hardy Weinberg Theory.

Forensic Statistics including random match probabilities and use of databases.

Explore non-human forms of DNA typing.

LO2 Demonstrate professional/practical skills by being able to perform and interpret selected practical applications of forensic DNA typing methods.

Demonstrate competence in;

Sampling and storing biological evidence from a variety of sources.

Performing a range of methods for extraction of DNA from trace samples.

DNA separation and detection methods.

Performing DNA typing methods and outline and evaluate the equipment requirements for performance of the test methods.

Importance of adherence to contamination avoidance procedures by using suitable PPE and utilisation of sterile consumables and experimental controls (negatives and positives where appropriate).

LO3 Analyse, evaluate and draw conclusions from data obtained in the laboratory and/or data presented in problem solving exercises.

Demonstrate competence in the correct interpretation of analytical results e.g. analysing electropherograms and sequencing chromatograms.

Interpretation of single source profiles.

DNA mixture interpretation

Y profiling and mitochondrial DNA profiling analysis, interpretation and reporting.

Data analytics using population databases and familiarity with the product rule, Nichols and Balding Size bias correction and agreed limits for court purposes.

LO4 Communicate scientific data in an appropriate format

Analyse, collate and report on experimental data generated in practical sessions.

Critically evaluate and analyse complex biological data.

Convey and communicate both orally and in writing, knowledge of Forensic DNA Typing.

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
65 %
End of Semester / Year Formal Exam
35 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Practical assessment Continuous Assessment UNKNOWN 50 % OnGoing 2,3,4
2 Continuous Assessment Continuous Assessment UNKNOWN 15 % OnGoing 1,3,4
             

End of Semester / Year Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Final theory examinarion Final Exam UNKNOWN 35 % End of Term 1,3,4
             
             

Full Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Tiered Classroom Lecture 2 Weekly 2.00
Laboratory Practical Science Laboratory Laboratory Practical 3 Weekly 3.00
Problem Based Learning Computer Laboratory Directed Learning 1 Weekly 1.00
Independent Learning UNKNOWN Self Study 8 Weekly 8.00
Total Full Time Average Weekly Learner Contact Time 6.00 Hours

Required & Recommended Book List

Required Reading
2018-06-18 Forensic DNA Typing Protocols Methods in Molecular Biology
ISBN 1493980890 ISBN-13 9781493980895

Preceded by Forensic DNA typing protocols / edited by Angel Carracedo. 2005.

Required Reading
2016-03-31 Forensic DNA Evidence Interpretation, Second Edition CRC Press
ISBN 1482258897 ISBN-13 9781482258899

Now in its second edition, this book is still the only one which covers the subject of forensic DNA interpretation from the standpoint of statistical analysis which is critical to supporting a legal claim. All chapters have been updated to reflect changes occurring in this fast-moving field, with special attention to PCR processes, worldwide values and the addition of internationally renowned scientists such as Peter Gill who is the father of DNA analysis.

Required Reading
2011 Advanced Topics in Forensic DNA Typing Academic Press
ISBN 9780123745132 ISBN-13 0123745136

John M. Butler

Required Reading
2014-10-22 Advanced Topics in Forensic DNA Typing: Interpretation Academic Press
ISBN 0124052134 ISBN-13 9780124052130

Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence Worked mixture examples illustrate the impact of different statistical approaches for reporting results Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011

Required Reading
2010 Fundamentals of Forensic DNA Typing Academic Press
ISBN 0123749999 ISBN-13 9780123749994

Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980's, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key

Module Resources

Non ISBN Literary Resources

All resources will be provided in Moodle.

Journal Resources

All resources will be provided in Moodle.

URL Resources

All resources will be provided in Moodle.

Other Resources

None

Additional Information

None