BIO09046 2013 Upstream Bioprocessing Practical
This module covers much of the practical knowledge, know-how and skills associated with the upstream cell-culture processing in a typical modern biopharmaceutical processing facility. The relevant learning is acquired through the completion of a sequence of laboratory-based and pilot-plant based practicals and experiments that are designed to impart the required knowledge, know-how and skills.
On completion of this module the learner will/should be able to;
Demonstrate a range of practical know-how and skills associated with relevant, lab-based upstream bioprocessing equipment for the Biopharmaceutical Processing industry.
Demonstrate a range of practical know-how and skills associated with relevant, pilot-plant based upstream bioprocessing equipment for the Biopharmaceutical Processing industry.
Interpret and critically evaluate the data and results obtained from the various upstream processing practicals.
Take responsibility for and direct the work of other individuals in a GMP- like environment.
Demonstrate research skill and capabilities in the overall upstream bioprocessing area.
Evaluate ongoing education and training needs in a continuous professional development environment.
Demonstrate effective communication skills both orally and in writing.
Module Assessment Strategies
As this is a practical-based module attendance at the NIBRT or IT Sligo training laboratories is a requirement. A certain amount of the assessment work will take place at these locations. The remaining assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQ’s/SAQ’s, project assignments/reports, practical/project work, essays, oral presentations, vivas etc.
The range of lab-based practicals covered may include some or all of the following items: aseptic techniques; cell culture techniques including cell counting (both manual and automated); sub-culturing including media exchange; thawing up and freezing-down of cells; benchtop bioreactor techniques such as disassembly/reassembly of parts; bioreactor instrument calibration; preparation of bioreactor for autoclaving; inoculation and operation of bioreactor; sampling and substrate analysis.
The range of pilot-plant based practicals covered may include some or all of the following items: P+ID (Piping and Instrumentation Diagrams) analysis work on bioreactor systems; CIP cycle operation on bioreactor systems; SIP cycle operation on bioreactor systems; cell culture run on the bioreactor vessel; operation of the perfusion skid system for the bioreactor vessel; calibration of pH and DO instruments; kla measurement on the bioreactor vessel under different operating conditions; aseptic sampling from the harvest vessel using the Novaseptum system; operation of the disc-stack centrifuge separating system; P+ID analysis work on the microfiltration skid system; operation of the microfiltration system; operation of the depth filtration skid system.
Coursework & Assessment Breakdown
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||Open Book Exam End of practical assessment exams.||Continuous Assessment||UNKNOWN||30 %||Any||1,2,3|
|2||Written Report Report on lab. practical work completed.||Continuous Assessment||UNKNOWN||20 %||Any||1,2,3,4|
|3||Individual Project Research project report and presentation.||Continuous Assessment||UNKNOWN||50 %||Any||3,5,6,7|
Part Time Mode Workload
|Lecture||Not Specified||Ongoing lectures.||1||Fortnightly||0.50|
|Independent Learning||UNKNOWN||Research project report and presentation.||3||Weekly||3.00|
|Laboratory Practical||Science Laboratory||NIBRT Training Facility attendance.||16||UNKNOWN||16.00|
Doran, Pauline M. 'Bioprocess Engineering Principles'. Academic Press. 1995.
Ratledge, Colin and Kristiansen Bjorn. 'Basic Biotechnology'. Cambridge Press. 2001.
Walsh, Gary. 'Biopharmaceuticals'. Wiley. 2003.