BIO09027 2011 DOWNSTREAM BIOPROCESSING ENGINEERING

General Details

Full Title
DOWNSTREAM BIOPROCESSING ENGINEERING
Transcript Title
DOWNSTREAM BIOPROCESSING ENG
Code
BIO09027
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
ASCI - Applied Sciences
Level
09 - NFQ Level 9
Credit
10 - 10 Credits
Duration
Stage
Fee
Start Term
2011 - Full Academic Year 2011-12
End Term
9999 - The End of Time
Author(s)
Deirdre Johnston
Programme Membership
SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201300 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201700 Master of Science in Biopharmaceutical Science SG_SBIOP_M09 201900 Master of Science in Biopharmaceutical Science
Description

This module aims to provide the student with a fundamental understanding of the principal calculations and equations that underpin downstream processing of the bioproduct. The goal is to promote the understanding of the development and use of working equations and theory pertaining to the isolation and purification of bioproducts produced in fermentors/bioreactors.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Describe different types of centrifuges and filtration equipment available for downstream processing.

2.

Estimate comparable settling conditions across different centrifuge types.

3.

Estimate permeate flux rates for cross-flow and dead-end filtration.

4.

Estimate time requirements for batch precipitation and crystalization.

5.

Estimate crystal size and yields for continuous crystalizers.

6.

Estimate the yield and purity of bioproducts following single and multiple stage extractions.

7.

Distinguish between different chromatography modes (IEX, HIC etc.).

8.

Predict breakthrough times during fixed bed adsorption.

9.

Predict the elution time, peak shape, resolution and yield of chromatography processes.

Indicative Syllabus

The following is a summary of some of the main topics included in this particular module: design types of centrifuges and filtration processes; Particle settling during centrifugation; Flux reduction due to cake formation during dead-end filtration ; Fouling and concentration polarization during crossflow filtration; Particle growth during precipitation and crystalization; Phase partioning during extraction; Isotherms describing solute adsorption ; batch versus continuous extraction and adsorption processes; design types of chromatography (IEX, HIC etc.); peak spreading and peak separation during elution chromatography; non-linear isotherm effects of peak shapes during elution chromatography. 

In addition, participants will be led through concise derivations of the working equations and example problems that illustrate use of the equations.  Specific discussions on minimizing the number of process steps by and choosing the optimum sequence of steps will be explored for different types of biomolecules from  antibiotics to antibodies.

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
100 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc. Continuous Assessment UNKNOWN 100 % OnGoing 1,2,3,4,5,6,7,8,9
             
             

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Not Specified Lecture 2 Weekly 2.00
Independent Learning UNKNOWN Self Study 7 Weekly 7.00
Total Part Time Average Weekly Learner Contact Time 2.00 Hours

Module Resources

Non ISBN Literary Resources

Harrison and Petrides, Bioseparations Science and Engeering, Oxford University Press

Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002

Belter, P.A. and Cussler, E.L. and Wei-Shou Hu, Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988

Other Resources

None

Additional Information
None