BIO09027 2011 DOWNSTREAM BIOPROCESSING ENGINEERING
This module aims to provide the student with a fundamental understanding of the principal calculations and equations that underpin downstream processing of the bioproduct. The goal is to promote the understanding of the development and use of working equations and theory pertaining to the isolation and purification of bioproducts produced in fermentors/bioreactors.
On completion of this module the learner will/should be able to;
Describe different types of centrifuges and filtration equipment available for downstream processing.
Estimate comparable settling conditions across different centrifuge types.
Estimate permeate flux rates for cross-flow and dead-end filtration.
Estimate time requirements for batch precipitation and crystalization.
Estimate crystal size and yields for continuous crystalizers.
Estimate the yield and purity of bioproducts following single and multiple stage extractions.
Distinguish between different chromatography modes (IEX, HIC etc.).
Predict breakthrough times during fixed bed adsorption.
Predict the elution time, peak shape, resolution and yield of chromatography processes.
The following is a summary of some of the main topics included in this particular module: design types of centrifuges and filtration processes; Particle settling during centrifugation; Flux reduction due to cake formation during dead-end filtration ; Fouling and concentration polarization during crossflow filtration; Particle growth during precipitation and crystalization; Phase partioning during extraction; Isotherms describing solute adsorption ; batch versus continuous extraction and adsorption processes; design types of chromatography (IEX, HIC etc.); peak spreading and peak separation during elution chromatography; non-linear isotherm effects of peak shapes during elution chromatography.
In addition, participants will be led through concise derivations of the working equations and example problems that illustrate use of the equations. Specific discussions on minimizing the number of process steps by and choosing the optimum sequence of steps will be explored for different types of biomolecules from antibiotics to antibodies.
Coursework & Assessment Breakdown
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc.||Continuous Assessment||UNKNOWN||100 %||OnGoing||1,2,3,4,5,6,7,8,9|
Part Time Mode Workload
|Independent Learning||UNKNOWN||Self Study||7||Weekly||7.00|
Harrison and Petrides, Bioseparations Science and Engeering, Oxford University Press
Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002
Belter, P.A. and Cussler, E.L. and Wei-Shou Hu, Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988