BIO09026 2011 UPSTREAM BIOPROCESSING ENGINEERING
This module aims to provide the student with a fundamental understanding of the principal calculations and equations that underpin upstream processing for the biopharmaceutical industry and to develop problem solving skills in these areas. The goal is to promote the understanding of the development and use of working equations and theory pertaining to the growth of cells and production of bioproducts in fermentors and bioreactors.
On completion of this module the learner will/should be able to;
Describe the fundamental techniques of genetic engineering and examples of their use for bioproducts.
Demonstrate an ability to estimate cell growth and product formation rates in both batch and continuous culture operations and also in fed-batch and perfusion cultures.
Estimate rates of enzyme-catalyzed reactions with and without mass transfer limitations.
Estimate the oxygen transfer coefficient for a variety of bioreactor configurations.
Analyse the probability of reactor contamination.
Describe the principal steps involved in the scale-up of a bioreactor.
Design bioreactors to minimise shear damage to cells and protein and also to optimise carbon dioxide exchange.
The following is a summary of some of the main topics included in this particular module: fundamentals of biochemistry, cell biology, microbiology and molecular biology for bioprocess operations; cell growth and product formation kinetics; heat sterilization, oxygen transfer and mixing; scaleup; alternate modes of operation (fed-batch, perfusion...); alternate bioreactor configurations; carbon dioxide exchange in bioreactors; shear damage in bioreactors. In addition, participants will be led through concise derivations of the working equations and example problems that illustrate use of the equations. Options for optimizing carbon dioxide exchange and minimizing shear in bioreactors will be evaluated.
Coursework & Assessment Breakdown
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc.||Continuous Assessment||UNKNOWN||100 %||OnGoing||1,2,3,4,5,6,7|
Part Time Mode Workload
|Independent Learning||UNKNOWN||Self Study||7||Weekly||7.00|
Shuler, M.L. and Kargi F., Bioprocess Engineering - Basic Concepts., Prentice-Hall., 2002
Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology., Wiley - Interscience., 1988
Bailey, J.E. and Ollis, D.F., Biochemical Engineering Fundamentals., McGraw-Hill International Editions, 1986
Prescott and Klein, Microbiology, McGraw-Hill, 2007