BIO09004 2008 PROTEIN PURIFICATION PROCESSING
This module examines all downstream processing aspects of a typical biopharmaceutical production process. It includes the various design factors that need to be considered for the design of an effective and efficient protein capture and purification process.
On completion of this module the learner will/should be able to;
Design an effective and efficient downstream process for a protein capture and purification application.
Outline and evaluate the various approaches to primary capture of the target material focusing primarily on affinity chromatography.
Describe the theory and practice of chromatography for purification applications.
Outline and evaluate the various chromatographic techniques available for purification and their different applications including relative advantages and disadvantages.
Interpret and explain other potential downstream processing technologies such as microfiltration, ultrafiltration, diafiltration, centrifugation, electrophoresis and viral exclusion techniques.
Outline and evaluate the main quality control and analytical techniques required for downstream processing.
Describe and critically evaluate the yield calculations for the various purification steps and their implications for overall process economics.
The range of topics covered will include some of the following: intracellular vs. extracellular protein in cell culture; the principles and operation of centrifugation; microfiltration, ultrafiltration, tangential flow filtration, diafiltration; electrophoresis; chromatographic techniques including gel filtration, ion-exchange (AEX and CEX), hydrophobic interaction, affinity-based techniques for both primary capture and protein purification; fluidised bed vs. packed bed design and column scale-up implications; yield calculations for chromatography steps; HETP and asymmetry in chromatography; viral exclusion technologies; quality control and analytical techniques for downstream processing.
Coursework & Assessment Breakdown
|Title||Type||Form||Percent||Week||Learning Outcomes Assessed|
|1||The assessment approach for this module will be 100% Continuous Assessment with a range of assessment methods employed including some of the following: short-form assessment exams incl. MCQs, project assignments, practical work, essays, oral presentations, vivas etc.||Continuous Assessment||UNKNOWN||100 %||OnGoing||1,2,3,4,5,6,7|
Part Time Mode Workload
|Independent Learning||UNKNOWN||Self Study||7||Weekly||7.00|
Walsh, Gary, Biopharmaceuticals., Wiley, 2003
Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988
Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.,
Amersham-Pharmacia. (www.amersham.com), 2000