BIO09002 2019 FERMENTATION AND CELL CULTURE PROCESS

General Details

Full Title
FERMENTATION AND CELL CULTURE PROCESS
Transcript Title
FERMENTATION AND CELL CULTURE
Code
BIO09002
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
09 - NFQ Level 9
Credit
05 - 05 Credits
Duration
Stage
Fee
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Mary Butler
Programme Membership
SG_SBIOP_M09 201900 Master of Science in Biopharmaceutical Science SG_SBIOP_O09 201900 Postgraduate Diploma in Science in Science in Biopharmaceutical Science SG_SBIOP_E09 201900 Level 9 Certificate in BioPharmaceutical Science
Description

This module examines  various upstream processing aspects of a typical biopharmaceutical production process. It includes the various design factors that need to be considered for bioprocess design. The primary focus will be on recombinant mammalian cell culture lines with some aspects of microbial lines also included.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Identify and evaluate  the main factors that are involved in the design of an upstream bioprocessing system including which cell line to choose for a particular processing application and the cell‑line storage conditions ‑ cell banking and cell culture scale‑up.

2.

Outline and evaluate the process kinetics relating to cell growth and productivity for a bioreactor process and the overall impact upon mass transfer balances in a bioreactor for air/oxygen and carbon-dioxide.

3.

Identify and interpret the basic mass and energy transfer details for   oxygen and carbon dioxide   within a bioreactor process.

4.

Evaluate and discuss the various designs of bioreactors available and their key features including relative advantages and disadvantages. Also describe the different options for media feeding systems.

5.

Describe the main aspects of bioreactor control and operation including the various instrumentation types involved.

Teaching and Learning Strategies

100% CA

Module Assessment Strategies

199% CA

Repeat Assessments

as per Q manual

Indicative Syllabus

The range of topics covered will include some of the following: cell line selection for optimal protein expression; cell banking and product scale‑up; media selection and nutritional requirements; the process kinetics of cell growth and replication;  ; basic mass transfer mechanisms for oxygen, and carbon‑dioxide  in a bioreactor; bioreactor design types such as stirred tank reactors, fixed‑bed, fluidized‑bed,  and the variety of single-use technology bioreactors; media feeding systems such as batch, fed‑batch, continuous, continuous with cell recycle and perfusion; bioreactor instrumentation and controls both online and offline; product yield and productivity  factors.  

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
100 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 MCQ Continuous Assessment Open Book Exam 10 % Week 4 1,2
2 MCQ/SAQ Continuous Assessment Open Book Exam 10 % Week 7 3,4
3 LAQ Continuous Assessment Closed Book Exam 30 % Week 10 1,2,3,4,5
4 Project report and presentation Project Written Report 50 % Week 14 1,2,3,4,5

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Not Specified Lecture 2 Weekly 2.00
Independent Learning UNKNOWN Self Study 7 Weekly 7.00
Total Part Time Average Weekly Learner Contact Time 2.00 Hours

Required & Recommended Book List

Required Reading
2013-04-29 Biopharmaceuticals John Wiley & Sons
ISBN 9781118687383 ISBN-13 1118687388

The latest edition of this highly acclaimed textbook, provides a comprehensive and up-to-date overview of the science and medical applications of biopharmaceutical products. Biopharmaceuticals refers to pharmaceutical substances derived from biological sources, and increasingly, it is synonymous with 'newer' pharmaceutical substances derived from genetic engineering or hybridoma technology. This superbly written review of the important areas of investigation in the field, covers drug production, plus the biochemical and molecular mechanisms of action together with the biotechnology of major biopharmaceutical types on the market or currently under development. There is also additional material reflecting both the technical advances in the area and detailed information on key topics such as the influence of genomics on drug discovery.

Required Reading
2006-05-25 Basic Biotechnology Cambridge University Press
ISBN 0521549582 ISBN-13 9780521549585

Biotechnology's wide-ranging, multi-disciplinary activities include recombinant DNA techniques, cloning, and the application of microbiology to the production of goods from bread to antibiotics. In this new edition, biology and bioprocessing topics are uniquely combined to provide a complete overview of biotechnology. A distinctive feature of the text is the discussions of the public perception of biotechnology and the business of biotechnology, which set the science in a broader context. This comprehensive textbook is essential reading for all students of biotechnology and applied microbiology, and for researchers in biotechnology industries.

Required Reading
2002 Bioprocess Engineering Pearson College Division
ISBN 0130819085 ISBN-13 9780130819086

This concise yet comprehensive text introduces the essential concepts of bioprocessing - internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information - to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications.

Module Resources

Non ISBN Literary Resources

Walsh, Gary., Biopharmaceuticals, Wiley, 2003

Ratledge, Colin and Kristiansen Bjorn, Basic Biotechnology, Cambridge Press, 2001

Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002

Journal Resources

Walsh, Gary., Biopharmaceuticals, Wiley, 2003

Ratledge, Colin and Kristiansen Bjorn, Basic Biotechnology, Cambridge Press, 2001

Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002

URL Resources

Walsh, Gary., Biopharmaceuticals, Wiley, 2003

Ratledge, Colin and Kristiansen Bjorn, Basic Biotechnology, Cambridge Press, 2001

Shuler, M.L. and Kargi, F., Bioprocess Engineering - Basic Concepts, Prentice-Hall, 2002

Other Resources

None