BIO08054 2019 Recombinant Cell Engineering

General Details

Full Title
Recombinant Cell Engineering
Transcript Title
Recombinant Cell Engineering
Code
BIO08054
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
08 - NFQ Level 8
Credit
05 - 05 Credits
Duration
Semester
Fee
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Tony McCabe
Programme Membership
SG_SMEDI_H08 201900 Bachelor of Science (Honours) in Science in Medical Biotechnology SG_SMEDI_K08 201900 Level 8 Honours Degree Add-on in Science in Medical Biotechnology
Description

The objective of this module is to introduce students to the recombinant drug discovery and development process. This module encompasses key steps in recombinant cell engineering and production including vector design and manipulation, gene delivery and expression, model system choice and expression detection. The module will further investigate approaches to enhancing recombinant protein expression and functionality. Concepts and methodologies uniquely associated with recombinant antibodies will be addressed. The module will also introduce gene-editing technologies and their role in future therapeutics.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Align the concept of rational drug design with recombinant protein production and identify the key tools required in the process.

2.

Assess the essential steps in the design, cloning and control of a recombinant expression systems.

3.

Describe methods employed to optimise nucleic acid sequences and cell expression systems for optimum protein expression.

4.

Examine methods of verifying the function and expression recombinant genes and gene products and verify the role of the gene/drug target in the disease process.

5.

Plan, design and execute laboratory protocols which are used to develop genetically modified cells.

6.

Analyse and report experimental concepts and data.

Teaching and Learning Strategies

Teaching and Learning Strategy

The module contact time will be split evenly between lectures and laboratory time.

Contact time will contain some tutorial elements which will be used to help students address problem topics and answer questions and provide feedback on assessments.

Lectures can comprise of elements such as: Powerpoint presentations, videos, articles for study and discussion on relevant topics.

The moodle platform will be used as a repository for course material.

Active Learning:

Students will be asked to read and contextualize relevant articles.

Students will be required to research and design experimental protocols.

Students will be required to research relevant information for the production of experimental reports.

Tutorials:

Occasional time will be set aside for tutorials will allow time for students to discuss the quizzes and assessments in a face to face format during which problems can be addressed and additional explanations provided. 

Small group writing tutorials will be provided for students who require additional help in constructing elements of reports (for example data presentation).

 

Laboratory work:

Students will plan and execute relevant experimental procedures within groups.

The laboratory classes are preceded by introductory lectures, so students are aware of the research question at hand, requirements, learning outcomes and any relevant health and safety concerns. Students are encouraged to raise learning issues, particularly with new techniques.

Module Assessment Strategies

Assessment Strategy

This module has an end of semester exam worth 50% and a laboratory element worth 50%.

The students’ performance will be evaluated through formative and summative assessments. 

 

Formative assessment:

The formative assessments may be delivered in class and through online quizzes so students can check their own progress. Assessments will be provided on an on-going basis throughout the semester at can be taken at a time of the students’ choosing. Typically they will be made available at the end of particular topics during the module. Online quizzes and review questions and crosswords are provided on the moodle platform, students can access these resources at any time once made available.

Quizzes will be designed in accordance to Bloom’s taxonomy, assessing elements relevant to the level 8 module status.

 

Summative assessment:

Module theory will be assessed through an end of semester exam worth 50% of the module. Relevant elements of the module theory, pertaining to testing for example, will also be assessed during practical evaluation, entailing short quizzes on the practical applications of the module. The laboratory element of the module (50%) will undergo a Practical Evaluation over 6 weeks of semester (or 26 hour equivalent). This evaluation will assess students’ basic laboratory skills and professional attitude, the students’ data presentation and analysis skills as well as their dissemination skills through submission of reports or individual report elements such as abstracts.

The moodle platform will be used by students to upload assignments and used in combination with Turnitin software.

Students, particularly overseas students,  may avail of remote proctoring of exams.

The student must reach an assigned mark in the final exam and achieve 40% overall to pass the module. The student must attend a minimum of 75% in the laboratory element of the module or they may be required to repeat and re-attend the module.

Repeat Assessments

If a student fails to achieve 40% in the module they will be required to resit the exam, resubmit or submit laboratory reports, write a theory assignment or a combination of these.  

Module Dependencies

Prerequisites
None
Co-requisites
None
Incompatibles
None

Indicative Syllabus

Align the concept of rational drug design with recombinant protein production and identify the key tools required in the process.

Rational versus irrational drug concepts.

Vectors, enzymes and reagents required for cell line development.

 

Assess the essential steps in the design, cloning and control of a recombinant expression systems.

Essential and optional vector features. Approaches to cloning genes and introduction to gene editing techniques.

 

Describe methods employed to optimize nucleic acid sequences and cell expression systems for optimum protein expression.

Promoter design and regulation. Effects of regulatory sequences in vector design and gene expression, including protein solubility. Determining factors in choosing cell model systems.

 

Examine methods of verifying the function and expression recombinant genes and gene products and verify the role of the gene/drug target in the disease process.

Gene, RNA transcript and recombinant protein detection. Confirmation of drug function and role in disease.

 

Plan, design and execute laboratory protocols which are used to develop genetically modified cells.

Chemical transfection and culturing of genetically modified cell lines.

 

Analyse and report experimental concepts and data.

Genetically modified cell line selection and expression analysis. Presentation of experimental data.

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
50 %
End of Semester / Year Formal Exam
50 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Quizzes Formative Assessment - % OnGoing 1,2,3,4,6
2 Laboratory work Practical Practical Evaluation 50 % OnGoing 3,4,5,6
             

End of Semester / Year Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Final exam Final Exam Closed Book Exam 50 % End of Semester 1,2,3,4
             
             

Full Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Lecture Theatre Theory delivery and tutorials 2 Weekly 2.00
Laboratory Practical Science Laboratory Laboratory work 2 Weekly 2.00
Independent Learning Not Specified Self Study 3 Weekly 3.00
Total Full Time Average Weekly Learner Contact Time 4.00 Hours

Required & Recommended Book List

Recommended Reading
2003-09-05 Biopharmaceuticals Wiley-Blackwell
ISBN UOM:39015056301230

This work provides a comprehensive overview of biopharmaceutical products. It discusses the method of production of these drugs, and the biochemistry/molecular mechanisms of action and biotechnology of all the major biopharmaceutical types.

Module Resources

Non ISBN Literary Resources

Biotechnology and Biopharmaceuticals: Transforming Genes into Drugs.  Author: Rodney, J.Y.Ho. Wiley-Liss 2003

Gene Cloning and DNA Analysis: An Introduction. Author Brown, T.A. Oxford Blackwell 2006

Drugs: From Discovery to Approval. Author: Rick, N.G., Wiley-Liss Press. 2004

Journal Resources

Specific articles will be provided to the students rather than recommending journals. For example:

https://www.pharmaceutical-journal.com/opinion/comment/rational-drug-design-identifying-and-characterising-a-target/10969751.article?firstPass=false

Newman, M., & Ausubel, F. M. (2016). Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology. Current Protocols in Molecular Biology, 31.4.1–31.4.6.doi:10.1002/cpmb.14 

https://bioprocessintl.com/2016/emerging-platform-bioprocesses-for-viral-vectors-and-gene-therapies/

Keles, E., Song, Y., Du, D., Dong, W.-J., & Lin, Y. (2016). Recent progress in nanomaterials for gene delivery applications. Biomaterials Science, 4(9), 1291–1309.doi:10.1039/c6bm00441e 

Other Resources

https://www.mendeley.com/guides/harvard-citation-guide

Podcasts:

https://speakingsciencepodcast.com/page/2/

Webinar:

https://www.bio-rad-antibodies.com/overcome-your-assay-challenges-with-our-free-webinar.html

https://www.youtube.com/watch?v=wOvpkJiVGhc&feature=youtu.be

https://www.youtube.com/watch?v=ct9gVN-KxHc

 

Videos:

www.dnai.org/lesson/go/23993/18682

http://www.dnai.org/lesson/go/23993/19236

http://www.dnai.org/lesson/go/23993/19245

 

Sciencedirect.com

Pubmed.com

NCBI.com

 

Additional Information

None