BIO08045 2019 CELL CULTURE PROCESSING

General Details

Full Title
CELL CULTURE PROCESSING
Transcript Title
CELL CULTURE PROCESSING
Code
BIO08045
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
LIFE - Life Sciences
Level
08 - NFQ Level 8
Credit
05 - 05 Credits
Duration
Semester
Fee
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Author(s)
Mary Butler
Programme Membership
SG_SBIOP_E08 201900 Level 8 Certificate in Science in Biopharmaceutical Science SG_SBIOP_K08 201900 Bachelor of Science (Honours) in Biopharmaceutical Science. SG_SBIOM_H08 202000 Bachelor of Science (Honours) in Science in Biomedical and BioIndustrial Sciences SG_SAPMS_H08 202000 Bachelor of Science (Honours) in Science in Applied Medical Sciences
Description

This module aims to provide learners with a deep understanding of the theory of mammalian cell culture processing as it pertains to the biosynthesis of modern biopharmaceutical products. It will examine the processing flow from cell vial thaw and subsequent scale-up through to protein synthesis and product formation. Consideration will be given to the development and characterisation of cell lines for use in biomanufacturing, the kinetics of cell growth and media requirements to optimise cell performance and productivity. The learner will be exposed to a detailed comparative discussion of mammalian cell bioreactor design, operation, and control for the successful manufacture of biotherapeutic proteins.

Learning Outcomes

On completion of this module the learner will/should be able to;

1.

Describe the fundamentals of mammalian cell biology and cell line development and explain the process kinetics relating to cell growth and productivity for a bioreactor process.

2.

Identify the key components of mammalian cell culture media formulations and relate those to the optimisation of a cell culture process.

3.

Compare and contrast the most common bioreactor designs available, their key features¸ and the relative advantages and disadvantages in different operating modes.

4.

Describe the key steps in bioreactor operation, the critical control parameters and the main operating and control loops associated with bioreactor performance monitoring.

5.

Analyse, evaluate and draw conclusions from data obtained from lab. demonstrations and/or data obtained from problem solving exercises.

6.

Perform research on a relevant topic and communicate in writing and orally an informed and evidence based analysis and evaluation of current thinking, with appropriate conclusions.

Teaching and Learning Strategies

as above

Module Assessment Strategies

Assessment Strategies

Continuous Assessment of Course Work - 30% of marks.

End of term online exam - 30% of marks.

Mini research project - 40% of marks.

The miniproject is a MANDATORY form of assessment for any modules on this programme with a miniproject component.

Repeat Assessments

as per Q Manual

Module Dependencies

Prerequisites
Fundermentals of cell biology
Co-requisites
None
Incompatibles
None

Indicative Syllabus

Indicative Syllabus

  1. Overview of cell biology relating to cell culture processing and bioreactor operations.
  2. Kinetics of cell growth, yield and productivities, cell counting techniques, cell density and cell viability measurements.
  3. Cell culture media design and formulation.
  4. Energy and mass transfer systems for bioprocessing.
  5. Bioreactor design and engineering.
  6. Bioreactor operation and control of critical parameters for protein synthesis.

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
100 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Open Book Exam Online Continuous Assessment UNKNOWN 15 % Week 4 1,2
2 Open Book Exam Online. Continuous Assessment UNKNOWN 15 % Week 9 3,4
3 Other Exam Online. Continuous Assessment Closed Book Exam 30 % Week 14 1,2,3,4,5
4 Individual Project Research and Problem Solving Continuous Assessment UNKNOWN 40 % Week 15 6

Part Time Mode Workload


Type Location Description Hours Frequency Avg Workload
Lecture Not Specified Online lecture 2 Weekly 2.00
Directed Learning Not Specified Course Notes. 4 Weekly 4.00
Independent Learning UNKNOWN Mini Research Activities. 4 Weekly 4.00
Total Part Time Average Weekly Learner Contact Time 6.00 Hours

Required & Recommended Book List

Required Reading
2007-08-13 Pharmaceutical Biotechnology John Wiley & Sons
ISBN 9780470012444 ISBN-13 0470012447

Offers a comprehensive introduction to the principles and concepts of pharmaceutical biotechnology.

Required Reading
2001-04-19 Basic Biotechnology Cambridge University Press
ISBN 0521779170 ISBN-13 9780521779173

Biotechnology: what it is, how it's used, and how you do it!

Required Reading
2002 Bioprocess Engineering Pearson College Division
ISBN 0130819085 ISBN-13 9780130819086

This concise yet comprehensive text introduces the essential concepts of bioprocessing - internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information - to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications.

Module Resources

Non ISBN Literary Resources

A variety of current topical peer-review papers will be provided via the virtual learning environment (VLE) module page for each lecture to illustrate current thinking on key issues.

Url Resources

Links to a variety of current topical peer-review papers will be provided for each lecture to illustrate current thinking on key issues.

 

'Bioprocess Engineering - Basic Concepts' by  Shuler,M.L. and Kargi F.

Prentice-Hall.  2002.   ISBN - 0-13-081908-5.

Journal Resources

as above

URL Resources

A variety of current topical peer-review papers will be provided via the virtual learning environment (VLE) module page for each lecture to illustrate current thinking on key issues.

Url Resources

Links to a variety of current topical peer-review papers will be provided for each lecture to illustrate current thinking on key issues.

Other Resources

None

Additional Information

None