BIO07011 2019 Protein Purification L7.

General Details

Full Title
Protein Purification L7.
Transcript Title
Protein Purification L7.
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
LIFE - Life Sciences
07 - NFQ Level 7
10 - 10 Credits
Start Term
2019 - Full Academic Year 2019-20
End Term
9999 - The End of Time
Paul Walsh
Programme Membership
SG_SBIOP_J07 201900 Bachelor of Science in Biopharmaceutical Science (Add-on) SG_SBIOP_E07 201900 Certificate in Biopharmaceutical Processing

This course aims to provide students with a broad understanding and practical appreciation of the main theoretical concepts and principles of protein purification (downstream processing). This will include both theory some relevant laboratory practicals.    

Learning Outcomes

On completion of this module the learner will/should be able to;


Outline the main elements of a downstream process design for protein capture and purification.


Describe the general theory and main principles of chromatography technology for protein purification applications.


Describe the general theroy and main principles of filtration technology for protein purification applications.


Describe the various viral exclusion and elimination technologies for downstream processing.


Perform a range of laboratory and pilot plant practicals relating to protein purification.


Communicate scientific information and data in an appropriate format and manner.

Teaching and Learning Strategies


Module Assessment Strategies

A combination of continuous assessment strategies, some laboratory experiments and an end-of-term exam.

Repeat Assessments


Indicative Syllabus

·         Introduction to Protein Purification.

·         The Design and Operation of an effective Protein Capture System.  

·         The Theory and Principles of Chromatography for Purification. 

·         The Theory and Principles of Filtration for Purification.  

·         Practical chromatography and filtration  applications for Purification.

·         Viral Exclusion Technologies for Protein Purification.  


Coursework & Assessment Breakdown

Coursework & Continuous Assessment
50 %
End of Semester / Year Formal Exam
50 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Continuous Assessment Short-form exams and lab. practical write-ups. Continuous Assessment Multiple Choice/Short Answer Test 10 % Week 5 1,2,3,4,5,6
2 Assessment by MCQ Online Continuous Assessment Multiple Choice/Short Answer Test 10 % Week 9 1,2,3,4,5,6
3 Report Format Continuous Assessment Written Report/Essay 30 % End of Semester 1,2,3,4,5,6

End of Semester / Year Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Final Exam Final Exam Closed Book Exam 50 % End of Term 1,2,3,4

Part Time Mode Workload

Type Location Description Hours Frequency Avg Workload
Lecture Distance Learning Suite Online lecture. 1 Weekly 1.00
Laboratory Practical Science Laboratory Laboratory practical experiments. 16 Once Per Semester 1.07
Directed Learning Not Specified Directed self-study. 5 Weekly 5.00
Total Part Time Average Weekly Learner Contact Time 7.07 Hours

Module Resources

Non ISBN Literary Resources

‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Wiley Publications.  2007.  ISBN - 978-0-470-01244-4.

'Bioseparations Science and Engineering' by R.G. Harrison, P.Todd, S.R. Rudge, D.P. Petrides.   Oxford University Press. 2003.  ISBN - 0-19-512340-9.

'Isolation and Purification of Proteins' by R. Hatti-Kaul, B.Mattiasson.

Marcel-Dekker.  2003.  ISBN- 0-8247-0726-5.

Journal Resources


URL Resources


Other Resources


Additional Information