BIO06012 2011 Downstream Bioprocessing Level 6.

General Details

Full Title
Downstream Bioprocessing Level 6.
Transcript Title
Downstream Bioproc Level 6.
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
ASCI - Applied Sciences
06 - NFQ Level 6
10 - 10 Credits
Start Term
2011 - Full Academic Year 2011-12
End Term
2019 - Full Academic Year 2019-20
Kieran Tobin
Programme Membership
SG_SBIOO_E06 201100 Certificate in Science in Biopharma Operations. SG_SGOOD_C06 201600 Higher Certificate in Science in Good Manuf Practive & Technol

This module aims to provide the student with a fundamental understanding of both the theory and practical aspects of protein purification processing as it pertains  to the manufacture of modern biopharmaceutical products.        

Learning Outcomes

On completion of this module the learner will/should be able to;


Describe the principal stages of downstream processing for protein products.


Outline the various approaches to primary capture of the target product.  


Describe the basic theory and practice of column chromatography for purification applications.


Explain other potential downstream processing technologies such as microfiltration, ultrafiltration, diafiltration,  centrifugation, and viral exclusion techniques. 


Perform a range of practical laboratory experiments relating to cell culture processing.

Module Assessment Strategies

Combination of continuous assessment techniques, laboratory experiments and end-of-term final exam.

Indicative Syllabus

The following is a summary indication of the main topics included in this module:

Intracellular vs. extracellular processes in cell culture processing and protein purification.

The fundamental principles and operation of centrifugation, microfiltration, ultrafiltration, tangential flow filtration, diafiltration.

The principles of column chromatography for bioseparation purposes.

The different types of chromatography available and their application: affinity, ion-exchange, hydrophobic-interaction, gel-filtration. The analysis of chromatograms and their main features.

Laboratory practicals associated with this module.

Coursework & Assessment Breakdown

Coursework & Continuous Assessment
70 %
End of Semester / Year Formal Exam
30 %

Coursework Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Continuous Assessment Short-form exams and laboratory experiments. Continuous Assessment UNKNOWN 70 % OnGoing 1,2,3,4,5

End of Semester / Year Assessment

Title Type Form Percent Week Learning Outcomes Assessed
1 Final Exam Final Exam UNKNOWN 30 % End of Term 1,2,3,4

Part Time Mode Workload

Type Location Description Hours Frequency Avg Workload
Lecture Distance Learning Suite Online lecture. 1 Weekly 1.00
Laboratory Practical Science Laboratory Lab. practical experiments. 20 Once Per Semester 1.33
Directed Learning Not Specified Directed self-study. 5 Weekly 5.00
Total Part Time Average Weekly Learner Contact Time 7.33 Hours

Module Resources

Non ISBN Literary Resources

‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988

Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.

Other Resources


Additional Information