BIO06012 2011 Downstream Bioprocessing Level 6.
Full Title
Downstream Bioprocessing Level 6.
Transcript Title
Downstream Bioproc Level 6.
Code
BIO06012
Attendance
N/A %
Subject Area
BIO - Bio Tech/Eng/Chem
Department
ASCI - Applied Sciences
Level
06 - NFQ Level 6
Credit
10 - 10 Credits
Duration
Semester
Fee
€
Start Term
2011 - Full Academic Year 2011-12
End Term
2019 - Full Academic Year 2019-20
Author(s)
Kieran Tobin
Programme Membership
SG_SBIOO_E06 201100 Certificate in Science in Biopharma Operations.
SG_SGOOD_C06 201600 Higher Certificate in Science in Good Manuf Practive & Technol
Description
This module aims to provide the student with a fundamental understanding of both the theory and practical aspects of protein purification processing as it pertains to the manufacture of modern biopharmaceutical products.
Learning Outcomes
On completion of this module the learner will/should be able to;
1.
Describe the principal stages of downstream processing for protein products.
2.
Outline the various approaches to primary capture of the target product.
3.
Describe the basic theory and practice of column chromatography for purification applications.
4.
Explain other potential downstream processing technologies such as microfiltration, ultrafiltration, diafiltration, centrifugation, and viral exclusion techniques.
5.
Perform a range of practical laboratory experiments relating to cell culture processing.
Module Assessment Strategies
Combination of continuous assessment techniques, laboratory experiments and end-of-term final exam.
Indicative Syllabus
The following is a summary indication of the main topics included in this module:
Intracellular vs. extracellular processes in cell culture processing and protein purification.
The fundamental principles and operation of centrifugation, microfiltration, ultrafiltration, tangential flow filtration, diafiltration.
The principles of column chromatography for bioseparation purposes.
The different types of chromatography available and their application: affinity, ion-exchange, hydrophobic-interaction, gel-filtration. The analysis of chromatograms and their main features.
Laboratory practicals associated with this module.
Coursework & Assessment Breakdown
Coursework & Continuous Assessment
70 %
End of Semester / Year Formal Exam
30 %
Coursework Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Continuous Assessment Short-form exams and laboratory experiments. | Continuous Assessment | UNKNOWN | 70 % | OnGoing | 1,2,3,4,5 |
End of Semester / Year Assessment
| Title | Type | Form | Percent | Week | Learning Outcomes Assessed | |
|---|---|---|---|---|---|---|
| 1 | Final Exam | Final Exam | UNKNOWN | 30 % | End of Term | 1,2,3,4 |
Part Time Mode Workload
| Type | Location | Description | Hours | Frequency | Avg Workload |
|---|---|---|---|---|---|
| Lecture | Distance Learning Suite | Online lecture. | 1 | Weekly | 1.00 |
| Laboratory Practical | Science Laboratory | Lab. practical experiments. | 20 | Once Per Semester | 1.33 |
| Directed Learning | Not Specified | Directed self-study. | 5 | Weekly | 5.00 |
Total Part Time Average Weekly Learner Contact Time 7.33 Hours
Module Resources
Non ISBN Literary Resources
‘Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.
Belter, P.A. and Cussler, E.L and Wei-Shou Hu., Bioseparations: Downstream Processing for Biotechnology, Wiley-Interscience, 1988
Amersham Pharmacia (GE Healthcare)., Series of Chromatography Manuals for industrial applications.
Other Resources
None
Additional Information
None